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Zika Outbreak Epicenter In Same Area Genetically-Modified Mosquitoes Released In 2015

Tyler Durden's picture




 

Submitted by Clare Bernishvia TheAntiMedia.org,

The World Health Organization announced it will convene an Emergency Committee under International Health Regulations on Monday, February 1, concerning the Zika virus ‘explosive’ spread throughout the Americas. The virus reportedly has the potential to reach pandemic proportions — possibly around the globe. But understandingwhy this outbreak happened is vital to curbing it. As the WHO statement said:

“A causal relationship between Zika virus infection and birth malformations and neurological syndromes … is strongly suspected. [These links] have rapidly changed the risk profile of Zika, from a mild threat to one of alarming proportions.

 

“WHO is deeply concerned about this rapidly evolving situation for 4 main reasons: the possible association of infection with birth malformations and neurological syndromes; the potential for further international spread given the wide geographical distribution of the mosquito vector; the lack of population immunity in newly affected areas; and the absence of vaccines, specific treatments, and rapid diagnostic tests […]

 

“The level of concern is high, as is the level of uncertainty.”

Zika seemingly exploded out of nowhere. Though it was first discovered in 1947, cases only sporadically occurred throughout Africa and southern Asia. In 2007, the first case was reported in the Pacific. In 2013, a smattering of small outbreaks and individual cases were officially documented in Africa and the western Pacific. They also began showing up in the Americas. In May 2015, Brazil reported its first case of Zika virus — and the situation changed dramatically.

Brazil is now considered the epicenter of the Zika outbreak, which coincides with at least 4,000 reports of babies born with microcephaly just since October.

zika-microcephaly

When examining a rapidly expanding potential pandemic, it’s necessary to leave no stone unturned so possible solutions, as well as future prevention, will be as effective as possible. In that vein, there was another significant development in 2015.

Oxitec first unveiled its large-scale, genetically-modified mosquito farm in Brazil in July 2012, with the goal of reducing “the incidence of dengue fever,” as The Disease Daily reported. Dengue fever is spread by the same Aedes mosquitoes which spread the Zika virus — and though they “cannot fly more than 400 meters,” WHO stated, “it may inadvertently be transported by humans from one place to another.” By July 2015, shortly after the GM mosquitoes were first released into the wild in Juazeiro, Brazil, Oxitec proudly announced they had “successfully controlled the Aedes aegypti mosquito that spreads dengue fever, chikungunya and zika virus, by reducing the target population by more than 90%.”

Though that might sound like an astounding success — and, arguably, it was — there is an alarming possibility to consider.

Nature, as one Redditor keenly pointed out, finds a way — and the effort to control dengue, zika, and other viruses, appears to have backfired dramatically.

zika

Juazeiro, Brazil — the location where genetically-modified mosquitoes were first released into the wild.

zika

Map showing the concentration of suspected Zika-related cases of microcephaly in Brazil.

The particular strain of Oxitec GM mosquitoes, OX513A, are genetically altered so the vast majority of their offspring will die before they mature — though Dr. Ricarda Steinbrecher published concerns in a report in September 2010 that a known survival rate of 3-4 percent warranted further study before the release of the GM insects. Her concerns, which were echoed by several other scientists both at the time and since, appear to have been ignored — though they should not have been.

Those genetically-modified mosquitoes work to control wild, potentially disease-carrying populations in a very specific manner. Only the male modified Aedes mosquitoes are supposed to be released into the wild — as they will mate with their unaltered female counterparts. Once offspring are produced, the modified, scientific facet is supposed to ‘kick in’ and kill that larvae before it reaches breeding age — if tetracycline is not present during its development. But there is a problem.

zika-mosquito

Aedes aegypti mosquito. Image credit: Muhammad Mahdi Karim

According to an unclassified document from the Trade and Agriculture Directorate Committee for Agriculture dated February 2015, Brazil is the third largest in “global antimicrobial consumption in food animal production” — meaning, Brazil is third in the world for its use of tetracycline in its food animals. As a study by the American Society of Agronomy, et. al., explained, “It is estimated that approximately 75% of antibiotics are not absorbed by animals and are excreted in waste.” One of the antibiotics (or antimicrobials) specifically named in that report for its environmental persistence is tetracycline.

In fact, as a confidential internal Oxitec document divulged in 2012, that survival rate could be as high as 15% — even with low levels of tetracycline present. “Even small amounts of tetracycline can repress” the engineered lethality. Indeed, that 15% survival rate was described by Oxitec:

“After a lot of testing and comparing experimental design, it was found that [researchers] had used a cat food to feed the [OX513A] larvae and this cat food contained chicken. It is known that tetracycline is routinely used to prevent infections in chickens, especially in the cheap, mass produced, chicken used for animal food. The chicken is heat-treated before being used, but this does not remove all the tetracycline. This meant that a small amount of tetracycline was being added from the food to the larvae and repressing the [designed] lethal system.”

 

Even absent this tetracycline, as Steinbrecher explained, a “sub-population” of genetically-modified Aedes mosquitoes could theoretically develop and thrive, in theory, “capable of surviving and flourishing despite any further” releases of ‘pure’ GM mosquitoes which still have that gene intact. She added, “the effectiveness of the system also depends on the [genetically-designed] late onset of the lethality. If the time of onset is altered due to environmental conditions … then a 3-4% [survival rate] represents a much bigger problem…”

 

As the WHO stated in its press release, “conditions associated with this year’s El Nino weather pattern are expected to increase mosquito populations greatly in many areas.”

Incidentally, President Obama called for a massive research effort to develop a vaccine for the Zika virus, as one does not currently exist. Brazil has now called in 200,000 soldiers to somehow help combat the virus’ spread. Aedes mosquitoes have reportedly been spotted in the U.K. But perhaps the most ironic — or not — proposition was proffered on January 19, by the MIT Technology Review:

“An outbreak in the Western Hemisphere could give countries including the United States new reasons to try wiping out mosquitoes with genetic engineering.

 

“Yesterday, the Brazilian city of Piracicaba said it would expand the use of genetically modified mosquitoes …

 

“The GM mosquitoes were created by Oxitec, a British company recently purchased by Intrexon, a synthetic biology company based in Maryland. The company said it has released bugs in parts of Brazil and the Cayman Islands to battle dengue fever.”

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Fri, 01/29/2016 - 18:19 | 7115555 NoWayJose
NoWayJose's picture

Two comments beyond the typical stuff -

Planned Parenthood likes this because it encourages abortions in a predominantly Catholic region.

Pregnant women from Central America can now plead 'asylum' to the US - and have their anchor babies here!

Fri, 01/29/2016 - 18:23 | 7115572 Mark Mywords
Mark Mywords's picture

My my my. Sounds like you've been eating too much tetracycline-laced shit, too.

Fri, 01/29/2016 - 18:53 | 7115704 The Juggernaut
The Juggernaut's picture

Are you sure the ZH newbies are ready for the hard truths, Tyler?

Fri, 01/29/2016 - 19:09 | 7115771 Father Thyme
Father Thyme's picture

Thank Bill Gates.

 

Bill gates releasing gm mosquitoes into crowd
www.youtube.com/watch?v=zIbvk2sDovU

Fri, 01/29/2016 - 19:21 | 7115823 quintago
quintago's picture

If nature can find a way to battle Zika, Nature can find a way to render the GM mosquito useless and turn it around to fux with you all modifiers.

Fri, 01/29/2016 - 19:23 | 7115830 Took Red Pill
Took Red Pill's picture

It's not nice to fool Mother Nature!

https://www.youtube.com/watch?v=ijVijP-CDVI

 

Fri, 01/29/2016 - 19:29 | 7115856 Nutsack
Nutsack's picture

So the mosquitos were released in an area with no Zika virus, and now there is Zika virus?

Fri, 01/29/2016 - 19:37 | 7115877 weburke
weburke's picture

NO<   

anyone hoping to live a good life will be smart to avoid ALL injections. 

Fri, 01/29/2016 - 19:56 | 7115931 Gaius Frakkin' ...
Gaius Frakkin&#039; Baltar's picture

If no leader in academia or finance is ever held accountable, don't expect them to understand the idea of unintended consequences.

This is the kind of shit you get when brainy no-common-sense types get together with rich douches who shouldn't have money to invest in the first place.

Fri, 01/29/2016 - 19:59 | 7115965 Al Tinfoil
Al Tinfoil's picture

Now. let's not be too critical of the rich and narcissistic who want to play God.

Fri, 01/29/2016 - 20:15 | 7116028 balolalo
balolalo's picture

THIS IS HOW YOU BREAK THE WORLD...

- don't go to the Latin America, you might catch Zika

- don't go to Africa, you might get Ebola, or AIDS

- don't go to SE Asia, you might get drug resistant malaria

- don't go to the Middle East because you might be blown up

- don't go to Europe you might get attacked by a migrant

- don't take a vacation or you might lose that job you hate that pays pennies

 

So what's left???

- sit at home

- spend spend spend

- pop pills, smoke meth, smoke dope, drink your self to death

- eat yourself to death

- fear others, fear new experiences

- destroy yourself and do as your fucking told

 

 

 

 

 

Fri, 01/29/2016 - 20:35 | 7116094 SMG
SMG's picture

I'm not sure this result from the GM mosquitoes is unintended.   The oligarch's goal is population control. So evil.

Fri, 01/29/2016 - 21:12 | 7116224 WTFRLY
WTFRLY's picture

Fuckin Joo World Order. Everyday.

Sat, 01/30/2016 - 03:50 | 7117020 Manthong
Manthong's picture

OK, so…

After I got over the malaria, TB, diphtheria and Ebola thing..

Now I have these Zika insects all over my body..

I am just glad I am not pregnant.. but what’s a guy to do anymore?

I think my head is shrinking, too.

 

..it's just a damn good thing that the deer I have sex with all the time don't have any ticks.

 

oh, my.. I have an idea for a screenplay..

"The Deer ___er"

Are De Niro and Walken still working?

 

 

Sat, 01/30/2016 - 09:00 | 7117304 Never One Roach
Never One Roach's picture

I do notice my lawn guy from the south has a very small head.

 

That's odd?

Sat, 01/30/2016 - 11:40 | 7117644 surfsup
surfsup's picture

Brazil. 95% vaccine rate. Why? Its Free... DTaP... Schedule? At 2, 4 and 6 months of age... Many similar side effects as on DTap insert...   http://www.scielo.br/scielo.php?pid=S0021-75572006000400002&script=sci_a... http://dx.doi.org/10.1590/S0021-75572006000400002 http://www.thepharmaletter.com/article/brazil-maintains-95-vaccination-c...

Sat, 01/30/2016 - 11:45 | 7117660 surfsup
surfsup's picture

So if this Zika is "really" the source of these deformations how come one can buy it freeze dried on line?  http://www.atcc.org/Products/All/VR-84.aspx?slp=1&geo_country=us#history...

Sat, 01/30/2016 - 11:47 | 7117667 surfsup
surfsup's picture

The deformations actualy reminds me of a sotry a few years ago about there being a two tier human in the future...  Normal humans and these bent over pigmy looking things...  was a serious story...  You woudn't think they'd???   nah......

Sat, 01/30/2016 - 11:51 | 7117683 surfsup
surfsup's picture

There is one time and only one time, humans are insanely predictable!  When you can convince them something is deadly and is spreading...   They all bite into it like children...   So is it normal to vax up a 2 month old, then another at four months and another at six months?  No one see's a problem potentially with that?  Oh yea... gotta get away from the big bad nasty thing that is deadly and spreading by shooting a baby up 3 times before they are even a 7 months old! Make sense!  Surely!  LMAO 

Sat, 01/30/2016 - 11:48 | 7117676 azusgm
azusgm's picture

The vaccine is now being given during pregnancy.

Sat, 01/30/2016 - 12:18 | 7117740 azusgm
azusgm's picture

Apparently the lawn guy has the intelligence to get up off the couch and go to work and get paid.

Sounds like a capitalist. Maybe he reads ZH right along with us.

Sun, 01/31/2016 - 01:50 | 7120204 Gonzogal
Gonzogal's picture

Since the "parent company" is in the UK, I tend to agree with you.

Fri, 01/29/2016 - 20:35 | 7116098 Xibalba
Xibalba's picture

- make sure you upgrade to Windows 10

Fri, 01/29/2016 - 20:57 | 7116177 Chuck Walla
Chuck Walla's picture

"If no leader in academia or finance is ever held accountable, don't expect them to understand the idea of unintended consequences.

This is the kind of shit you get when brainy no-common-sense types get together with rich douches who shouldn't have money to invest in the first place."

Pretty much describes the Obama regime.

FORWARD SOVIET!

Fri, 01/29/2016 - 20:10 | 7116012 Mentaliusanything
Mentaliusanything's picture

Just walking around in Brazil might be enough. Men covered in yellow hazmat suits, top to toe using blowers discharging something that is quite clearly dangerous because of their dress....... around normal people walking in normal attire.

It means ladies and gentleman the human logic has dissolved to the point that they have developed the brains of a smashed crab.

 

Fri, 01/29/2016 - 20:43 | 7116123 lincolnsteffens
lincolnsteffens's picture

Mission accomplished.

Sat, 01/30/2016 - 04:08 | 7117070 californiagirl
Fri, 01/29/2016 - 19:10 | 7115781 Paveway IV
Paveway IV's picture

Bug guys/gals: VDU's blog has this article: Zika virus disease samples...don't pass urine (by).. on PCR being superior against urine samples vs. blood samples. The graphs show a Cycle Threshhold of 38. Does that mean repeating the amplification procedure 38 times, or does that mean amplifying the RNA 38x (with presumably less than 38 separate 'procedures'). How long does it take to do a test like that once you have the sample in front of you? Sorry if that's not the precise PCR terminology - I'm more familiar with my meth lab blue ice bakes chemistry than PCR. 

Fri, 01/29/2016 - 22:19 | 7116478 unicorn
unicorn's picture

this PDF might interest you paveway. although its in german, its one of the best introduction into how GMO is done. its about engineering plants, but the techniques are also used for any other living being.

Type Ctrl F and type "Tabelle 11" or go to page 104, there you ll find a most useful list of techniques and if they can be detected, if you dont know what they put in exactly. the result is "no"

this is the source:

https://www.zh.ch/internet/baudirektion/awel/de/biosicherheit_neobiota/g...

here the PDF direct:

http://www.awel.zh.ch/internet/baudirektion/awel/de/biosicherheit_neobio...

he describes how stuff happens you cant predict, when you do a genetic modification, anything goes. latent virus can be woken up for example etc.

releasing GM animals, you cant control with modifications, you cant control is not very clever, but surely gives a nice profit, if oxitec can sell now their new generation of GM Chimeras to eliminate the problem, they probably created, and then, who knows, again another one, or the vaccine, or whatever....... and reducing population in one clap.

not that some people didnt do this kind of business before...

but maybe they just wanted to do a great thing for GMOs, they desperately need good propaganda. and as they are, not thinking much further then short profit, they suppressed the sideffects. search seralini or pusztai, and you see, how serious scientifics get silenced.

helas, dangerous sh*t goin on these days.

 

 

 

Sat, 01/30/2016 - 01:38 | 7116889 Paveway IV
Paveway IV's picture

Thanks, nicorn. I need to find a free program for translating .pdf files without having to cut and paste.

Sat, 01/30/2016 - 18:02 | 7118997 . . . _ _ _ . . .
. . . _ _ _ . . .'s picture

Try this:

https://translate.yandex.com/

Put in the url and select 'site' as well as the input and output languages.

It will translate any web page/pdf.

Sun, 01/31/2016 - 11:54 | 7120935 Paveway IV
Paveway IV's picture

I was pissed because I still couldn't get this to work on the .pdf, sos. Then I actually tried following your directions and clicked on 'site'. Works like a charm on the German .pdf unicorn linked above. Thanks.

Fri, 01/29/2016 - 23:37 | 7116654 tarsubil
tarsubil's picture

How long does it take? They have super fast and stable polymerases which make the reaction quick. You could do 38 cycles in less than an hour. It depends on how big your target is and how fast your polymerase is.

Anywho, this is all academic. Not sure it matters if it can be found in urine quicker than in blood. I'm not sure about this mosquitoe story either. Things have just gotten weird. 

Now that I think about it. Zika has been around for a while but it has not definitely been shown to cause this birth defect. The evidence seems to be in this situation. Wouldn't it be tragic if the mosquitoes were the thing causing the birth defect and zika has a correlation because it is spread by franken bug? Meh, that's probably not what's going on. Just thought I'd add to the fire.

Sat, 01/30/2016 - 01:34 | 7116880 Paveway IV
Paveway IV's picture

Thanks, tarsubil. That answers my question - I was wondering if it was merely a matter of leaving it in 'the soup' as it were for a longer time to amplify it.

The urine PCR would be of great interest to pregnant women who are unsure if they were exposed. From what I understand in the VDU article, blood PCR testing needs to happen within four or five days of the onset of symptoms. After that point, testing will not detect (or be poor at detecting) exposure to the virus. It would be nice for doctors to have a window of ten or fifteen days after onset for initial testing or confirmation of exposure from urine as an alternative.

"...Wouldn't it be tragic if the mosquitoes were the thing causing the birth defect and zika has a correlation because it is spread by franken bug? Meh, that's probably not what's going on. Just thought I'd add to the fire..."

A perfectly reasonable assumption that scientists need to consider, but what pregnant woman would wait for scientists to confirm this? God only knows what kind of crap they're spraying around Africa and South America to control these mosquitoes. Chlorpyrifos and DDE (metabolized from DDT) are both associated with microcephaly, as well as many other organophosphate-based insecticides. Mosquito control may be more lethal than Zika. Then there's the problem of what if it's some completely unknown virus that just happens to be found in about the same areas as Zika is found. Scary. As if pregnant women don't have enough to worry about.

Sat, 01/30/2016 - 01:59 | 7116918 Dantzler
Dantzler's picture

rtPCR being superior in urine vs. blood would imply that the target (Zika) sequence is more prevalent in urine vs. serum. Each cycle roughly doubles the signal from the starting number of target sequences. If target is not present or extremely rare, little signal amplification occers. 2^38 = 275 billion (274,877,906,944) so each copy of target (Zika) sequence could not be amplified more than this much after 38 cycles (the efficiency is < 100% per cycle.) This magnitude of signal would be detected. A Positive result is usually observed after 20-25 cycles (2^25 = 33,554,432, or 0.012% of the potential amplification @ 38 cycles) or many, many cycles less than 38 is sufficient to detect a typical positive. All these data say is that either urine is a much better source of Zika RNA and it has a longer detectable half life in this compartment or maybe their serum assay needs optimizing.  If I squint, it seems like the urine Zika RNA levels may have a double peak at 5 & 10 days and then they drop off. Usually rtPCR experiments normalize the # cycles to detect the unknown analyte vs. how many cycles a set of control primers take to detect a known target (aka "housekeeping gene that is always present in a predictable amount.) rtPCR measures messanger RNA present by first converting it to cDNA and then doing PCR on that. An rtPCR reaction takes 2 hours (to do 40 cycles, more than that is futile) once you have collected all the samples and reagents, etc. and hit "GO" on the thermocycler. HTH.

 

This article is sensationalist tripe. Zika is a virus that was first discovered in the Zika forest in Uganda 1947. Yes, it is transmitted by mosquitos. Yes, there are a metric fuck ton of mossies in that area of Brasil. The company's strategy was to release a bunch of modified male mossies  so they would mate with the local wild type females (and thereby prevent them from beng fertilized by a wild type male) and the modification would render the resulting larvae non-viable, thus reducing the overall rate of that species of mosquito's reproduction. Tetracycline can be used to control gene expression. My guess us that the modified males have a gene not required in adults but critical for larval development under the control of the Tet promoter. See:

https://en.wikipedia.org/wiki/Tetracycline-controlled_transcriptional_ac...

In the absence of tetracycline, the larva doesn't make the critical gene product and kablewie! No more larva. Tetracycline is used as a selection agent in lab because its hard to do research on mossies when the little bitches keep making inviable offspring.

Things got interesting because the GM scientists didn't do their due diligence to ask "what is the level of tetracycline out there in our test environment relative to levels that can drive "enough" gene expression of the ciritical gene? Thus the whole point of this story is "Oh fuck, we're not killing nearly as many bad mossies as we thought!" Colombia & Venezula are also reporting outbreaks. Were there similar GM mossies released in these areas...? More to the point, what is the variability in the viral genome over time and locale, and can it be attributed to the typical rate of viral "sequence drift" under such conditions? A GM virus would be pretty easy to spot by a competent virologist, given enough data.

 

This article sucks monkey cock because the non-discerning ZH reader these days will notivce "genetically modified", "Zeka", and "mosquito" in the same sentance and start squeeling about evil corporations conspiring to release biological weapons against their unsuspecting citizens (hint: they only do that in San Francisco and south Dorset ;)

https://en.wikipedia.org/wiki/Operation_Sea-Spray

Sat, 01/30/2016 - 02:40 | 7116980 Paveway IV
Paveway IV's picture

Most informative, Dantzler. Thanks for all the detail. Follow up question from the peanut gallery: If zika can be detected after 20-25 cycles, but may take as many as 38 cycles to be detectable (if only a small number were originally present), then when do you decide when to stop the amplification and look for the zika RNA - or I guess Zika cDNA in this case? Can you interrupt the amplification to test the sample, then continue to cycle it to amplify it more later on? You mentioned thermocycler - what kind of temperatures are involved to make the magic happen? Can I make my own thermocycler out of, say, a used water heater I have in the garage? No - scratch that. It leaks and I use it for my meth cooks anyway.

The environmental tetracycline thing, as I understand it, simply means that there's a potential reason why some of their GM mosquitos from the farm in Brazil will not die early as planned. Yet they were claiming success at the end of last year I think. Mosquito populations were dropping, and dengue fever and various other mosquito-borne virii illnesses were down. So I'm not seeing the connection to Zika here either unless there was some unintended consequence of another species of mosquito proliferating without the competition from the ones targeted by the GM frankensquitos. So aside from the few GM mosquitos that get away from the farm, they really shouldn't be a factor considering the bazillion other mosquitoes in Brazil. I would think bats would be a pretty good natural vacuum cleaner for the mosquitos, but I understand they've killed off a considerable number of the bats with insecticides meant to control the mosquitos. <sigh...>

I'll watch VDU's blog to see when someone sequences the Zika and makes some determination about how the genomes compare across geography/time. I don't understand most of it even when I'm sober, but it's fascinating that they can even do such a thing and map out the way it spread. 

Sat, 01/30/2016 - 03:49 | 7117045 Dantzler
Dantzler's picture

Each rtPCR cycle is not perfect. Not all copies of target get duplicated each cycle. Sometimes errors occur that cause signal to accumulate even though it is not driven by amplification of the target. Eventually, you will get a positive signal no matter what. 40 cycles is a pretty typical cycle # to stop at, beyond which you're looking at the accumulated errors that fuck with your signal. The authors in that blog post are reaching to use 38 cycles as a cutoff, but these assays have probably been thrown together and have not had a lot of time to optimize (also note the lack of controls to normalize signal to, in other words, did my mRNA -> cDNA conversion work, did my assay work, etc.) If the mRNA to cDNA converstion does not produce a representative pool of cDNAs then that is also a problem that could cause lower sensitivity. It is pointless to interrupt the assay. If it doesn't give you a positive signal well before 40 cycles, then your sample is negative or you need to work on your assay conditions.

Temperatures vary, but in general the denaturing step where the double stranded DNA is 'melted' to 2 single strands of DNA is done near boiling (94-98C.) This is followed by annealing (50-65C,) where you want it to be cool enough for your probes to bind to the target DNA sequence (e.g. a viral specific gene), but hot enough to eliminate non-specific binding to sequences that are very similar to but not the same as target DNA sequence. Annealing temp is a function of your primer sequences; longer primers and more G-C rich primers can anneal properly at higher (more stringent) temperatures due to the increased binding energy associated with longness and G-C richness. This varies with assay, but lower annealing temperatures give you more non-specific amplification (i.e. false positives.) Finally once the primers are annealed, an extension temperature is held for a long enough time for your particular polymerase to synthesize the length of traget DNA spanned by your primer pair. This is typically 75-80C.

Some PCR reactions only use 2 temperatures where annealing and extension are combined into one step. I don't really do a lot of PCR, so specific parameters may vary for the rtPCR application, but this is the general idea.

https://en.wikipedia.org/wiki/Polymerase_chain_reaction

Kary Mullis once gave a talk at a biotech I worked at. Brilliant, eccentric dude. Loved to surf and occassionally consume LSD. He got sweet fuck all from the company he worked at for inventing a technique that completely revolutionized what we can do with nucleic acids in still untold ways.

You can do PCR at home. You will need to have your primers synthesized by an entity that can do that economically. Primer design is not trivial, but there are online tools to help. I can't comment on the particular special labelled primers you need for rtPCR or the specialized equipment to detect product accumulation, but that won't be as cheap as say bulking up some insert DNA or doing minor targeted mutagenesis where your product is just plain Jane DNA and not a label. You can do it manually with 2-3 heat blocks or water baths that are accurately temperature controlled, you could make one of these for $650, or you could troll ebay & dying biotch company auctions, etc. for used (maybe even broken if you are handy) thermocyclers.

http://openpcr.org/

Other good things to have are micropippets and tips, a non-frost free freezer (i.e. does not temperature cycle), and mol bio grade water, buffers, etc. A biological safety hood is also very handy both to keep you safe from your samples and equally if not more to keep your samples safe from you (and the general stuff that is floating around labs, etc.)

You are spot on with regard to the utility of a test that could inform women if they are infected (the disease is often sub-clinical in presentation and thus can be easily missed due to absence of malaise). The problem is that rtPCR is generally expensive, requires specialized reagents and equipment & reliable electric power, and generally is not easy to do in resource poor areas.

Sat, 01/30/2016 - 14:47 | 7118281 Paveway IV
Paveway IV's picture

Thanks again for the great replies, Dantzler.

"...The problem is that rtPCR is generally expensive, requires specialized reagents and equipment & reliable electric power, and generally is not easy to do in resource poor areas..."

I don't expect Brazil to ever do much of anything regarding this testing, even if they had the capability. The authoratative medical research that led to the Brazilian government making the connection were little more than 'guesses' from the same Brazilian government health officials that were bribed to encourage pregnant Brazilian women to take an unnecessary and off-label dose of TDAP(Tetanus, diphtheria, and acellular pertussis) vaccine meant for older children. The decision to encourage that was made in 2014 but not ramped up until last summer when stocks became widely available, initially in... wait for it... northeast Brazil.

So naturally, any scientific mind would immediately associate the rapid increase in northeast Brazilian birth defects with a relativly benign, obscure, difficult-and-expensive-to-detect virus that has never been known to have caused those birth defects anywhere else on earth. I'm sure this will be solved with a mandatory TDaP+Z vaccine. At least that's my tin-foil-hat-nutter prediction.

Tdap is manufactured by two pharmaceutical companies: Sanofi Pasteur of France and GlaxoSmithKline (GSK) of the United Kingdom. God bless them - they are heros. Company executives would be wise to avoid nail-guns while in Brazil.

Sat, 01/30/2016 - 15:01 | 7118352 Miffed Microbio...
Miffed Microbiologist's picture

Agree with everything you have said. Personally I have found any ct in influenza above 38 to not be repeatable and therefore dubious. Whether this represents an early infection or recovery is left to the physician to decide clinically. Also, without an internal control, any negative result would possibly represent inhibition and the result would be meaningless. I see this many times in clinical samples that were not found in the development of the assay. Dilution can help but you run the risk of losing your target.

I would be curious to see the analytical range and the lower limit of detection and/or quantification in the method used. Plus the use of an amperase to eliminate reamplification of amplicons which would represent a serious risk of contamination in the lab. A personal grief I experience daily in new assays.

Miffed

Sat, 01/30/2016 - 15:35 | 7118468 Dantzler
Dantzler's picture

Thanks for a clinicians perspective, Miffed. I had not heard of amperase, so I need to look that up. I work on developing diagnostics, but occupy more the protein side of things. In this area, rtPCR is a very powerful technique and it is always nice to be able to get data from multiple, orthogonal assays. I recently came across a case where rtPCR was negative because the patient had cleared parasite, but the protein biomarker hung around for a few weeks afterwards in blood at levels sufficient to give a positive result in a lateral flow assay.

Always a pleasure to meet you in the comments section here.

Sat, 01/30/2016 - 16:35 | 7118709 Miffed Microbio...
Miffed Microbiologist's picture

And the pleasure is all mine for the experience. Any intellectual discussion on such a topic between research and clinical is always rewarding. I have the fortune learning PCR in the early days when it was plagued with problems of contamination. I think the Nested PCR protocol for CMV we were working on has taken years off my life. My response to management " burn down this damn lab and build me another." was not taken in the humorous spirit I had intended. rtPCR has been a God send in speed once specificity has been attained.

My experiences in the field has had an effect on adjustment of primers and thermocycling times. We in the field can provide critical data in this area. I called one vender, claiming a 40 ct was valid for their influenza A but I found it not repeatable and refused to report them as positive to clinicians. One year later they admitted I was right and retracted to 38. Also we have to deal with the physicians on the other end. I recently had to question a dr claiming treatment failure when his patient has positive GC PCR after one month of treatment. I told him this could be the result of residual DNA and a culture would be more meaningful than PCR which would show the viability of the organism as well as susceptibility to rule out extreme resistance.

Hat tip to you and all the other ZHers who are looking at this rationally and scientifically and not being manipulated by fear to come to some frightfully erroneous conclusions. Recently I have found some arrogantly espousing actions without such considerations and instead of questioning their assumptions they found it more advantageous to personally attack me. It is a joy for me to see not all here have descended into such a state. I am humble to know I don't know all and welcome new knowledge you and others can bring me.

In friendship,

Miffed

Sat, 01/30/2016 - 06:01 | 7117155 WojtekSz
WojtekSz's picture

piv:

JimStone freelance is calling the Zika virus a scam and provides some data on this - especially very disturbing coincidence of TDAP vaccines introduced for pregnant in Brasil before week 42nd. When those mothers gave birth the mikrocephaly heas suddenly outburst in numbers

have a look at this http://82.221.129.208/ifyouareinamericayouprobablycantseethisk3.html

he may seem a little strange to many of you but check his several notes on zika before you make out your mind. Technically his website looks vary bad but he keep it this way so he can defend it against many who would be interested to put it down.

if in doubt about his competences check his fukushima report...

Sat, 01/30/2016 - 09:41 | 7117370 I need more asshats
I need more asshats's picture

I can see it. Jim asking for donations! He needs some corporate backers.

Anyway Zika is a nonevent it just lasts a few days in the breeding humans body. So stay inside a few days then do your filthy dirty sex acts then you are a safe breeder.

Fri, 01/29/2016 - 19:12 | 7115785 FreeMoney
FreeMoney's picture

Where is the CIA funded lab with the military advisors?  Ukraine?

Sat, 01/30/2016 - 18:09 | 7119014 . . . _ _ _ . . .
. . . _ _ _ . . .'s picture

And Georgia and Kazakhstan and Dubai and UAE and who knows where else...

Black sites.

Fri, 01/29/2016 - 20:02 | 7115977 Pipetex
Pipetex's picture

Come on... we have been detected already

 

http://www.shtfplan.com/headline-news/here-is-the-list-of-web-sites-you-...

 

Check the last name on the list!

 

 

Fri, 01/29/2016 - 21:48 | 7116362 MontgomeryScott
MontgomeryScott's picture

I like the original list, as well as the 'ignored site' list.

Actually, I have 10 of the 20 sites below 'favorited' already.

"We are somewhat outraged that several top alternative news and information web sites failed to make this list, thus we have included a short list below."

Fri, 01/29/2016 - 22:28 | 7116506 azusgm
azusgm's picture

Trunews.com has some interesting guests. They have recently hired reporters to create original content. They're growing. Their admiration for Obama and Hillary Clinton would fall in line with how Rev. Manning feels about Obama. I feel right at home.

Fri, 01/29/2016 - 23:56 | 7116735 TheEndIsNear
TheEndIsNear's picture

These are the websites the government will shut down first.

Sat, 01/30/2016 - 00:11 | 7116766 Innominate
Innominate's picture

Lew Rockwell is on that list?!?! The chap behind the Mises Institute is on that list?!?!

They must truly fear the Austrian school of economics. Can't have the peasants learning real economics or they might just start repudiating the paper scams of the bandit class.

Do NOT follow this link or you will be banned from the site!